replacement mutant meaning in English
置换突变型
Examples
- Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b , in which the yields of avermectins b in 82 isolates were only about 0 . 1 ~ 2 % of those produced by the parental strain olm73 - 12 . 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis . the mutant only producing avermectins bl was not detected
摇瓶发酵和hplc分析结果表明,有99个突变株仍然产阿维菌素b的4个组分,其中82株的发酵单位很低,仅为出发菌株olm73 - 12的0 . 1 2 ,从中挑取2株经pcr扩增和测序验证,均发生了正确的基因取代;没有检测到仅产b1的突变株,这表明阿维菌素b2组分的产生并不是因为阿维菌素pks上dh2的部分活性所造成。